Study of the Antioxidant Activity and Total Phenolic Content of Crude Extract from Michelia alba Flower

Charinthorn  Fakkham; Anongnooch  thumpad; Phanthipha  Phuttamek; Salinthip  Kunsilarak; Saengsit  Kritsadee

Charinthorn Fakkham Suan Sunandha Rajabhat University, Thailand
Anongnooch thumpad Suan Sunandha Rajabhat University, Thailand
Phanthipha Phuttamek Suan Sunandha Rajabhat University, Thailand
Salinthip Kunsilarak Suan Sunandha Rajabhat University, Thailand
Saengsit Kritsadee Suan Sunandha Rajabhat University, Thailand

Keywords: Michelia alba, Antioxidant, Free radical, Total Phenolic Content

Abstract

Antioxidants decrease the amount of free radicals and reactive oxygen species in vivo. Herbal medicines have been reported for their significant antioxidant activities. Michelia alba (M. alba) is a flowering tree best known for its essential oil, which has been used for a long time as a fragrance ingredient in perfumes, cosmetics, and traditional medicine. The objectives of this study were to determine the antioxidant activity and the total phenolic content of the flower M. alba, which was extracted by maceration with 95% ethanol. The antioxidant test was used 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging method and total phenolic compounds by the Folin-Ciocalteu method. The antioxidant activity results of M. alba by the DPPH method showed the antioxidant activity IC50 at 0.035 mg/ml. The total phenolic content was 98.85 ± 0.04 mg GAE/mg of dry extract. In conclusion, the results of this study demonstrated the antioxidant activity and contained phenolic compounds and could potentially offer health benefits.