STUDY GROWTH CURVE AND SOME FACTORS THAT EFFECT TO RED PIGMENT PRODUCING OF RED YEASTS.

Tanakwan  Budsabun; Wattana  Panphut; Kitthisak Khlaeo  Chansukh

Tanakwan Budsabun Department of Industrial Microbiology, Faculty of Science and Technology, Suan Sunandha Rajabhat University, Bangkok, Thailand
Wattana Panphut Department of Industrial Microbiology, Faculty of Science and Technology, Suan Sunandha Rajabhat University, Bangkok, Thailand
Kitthisak Khlaeo Chansukh Department of Applied Thai Traditional Medicine, College of Allied Health Sciences, Suan Sunandha Rajabhat University, Bangkok, Thailand

Keywords: Red yeast, Red pigment, Optimal culture conditions

Abstract

The three isolates of Red yeast, isolate B, isolate G and isolate Hwere screen from soil that collected in Dusit province, Bangkok, Thailand. This study was direct to study some optimal culture condition for red pigment production by Red yeasts isolate B, G and H under liquid medium in 500 ml Durham bottle culture. The growth curve of three isolates were study. The effect of four carbon sources, glucose, sucrose, glycerol and fructose and aeration by shaking at 150 rpm and 190 rpm on red pigment production by Red yeasts were test. The result found that aeration by shaking at 190 rpm, all of three isolates reached to stationary phase faster than aeration by shaking at 150 rpm. Aeration by shaking at 190 rpm all of three isolates reached to stationary phase at 48 hrs and aeration by shaking at 150 rpm all of three isolates reached to stationary phase at 72 hrs. For the effect of carbon sources and aeration on red pigment production, glucose 10 grams per liter was the best carbon source follow by sucrose, glycerol and fructose respectively and aeration by shaking at 150 rpm was the best condition for all the three isolates.