IN SILICO CHARACTERIZATION OF SINORHIZOBIUM AND MESORHIZOBIUM AMYLASES.

Abstract

Microbial amylases are the dominant enzymes in industrial markets. These enzymes hydrolyze the starch molecule to the glucose unit polymers. A large numbers of amylases were obtained from diverse species of microbes. Starch as the main substrate for these enzymes which plays the important role in human diet obtains from many economically important crops such as wheat, rice, tapioca and potato. The final products of maltodextrin, modified starches, or glucose and fructose syrups were obtained by this enzymatic reaction. As there are large numbers of amylases from different microbial sources for different applications, the molecular characterization of the enzyme is vital for the researchers and producers. This research paper provided the comprehensive in silico information on amylases from two distinct rhizobia genera Sinorhizobium and Mesorhizobium. Thermostability and pH stability of amylases from Sinorhizobium and Mesorhizobium genera as well as the genomic distribution, physicochemical and structural features were analyzed. The physicochemical characterizations such as grand average hydropathy (GRAVY), aliphatic index (AI), extinction coefficient (EC), isolelectric point (pI), and instability index (II) were evaluated. S-S bridges, secondary structures and their homology modeling of these enzymes were presented with PyMol model visualization. This research finding could provide the analytical information for isolating and producing these enzymes for industrial purposes.