EVALUATION OF THE IN VITRO ANTIOXIDANT CAPACITY OF INTHAJAWORN RECIPE A THAI TRADITIONAL CANNABIS MEDICINE AND PHYTOCHEMICAL ANALYSIS BY HPLC AND GCMS TECHNIQUES

Chawalit  Yongram; Panupan  Sripan; Suwadee  Chokchaisiri; Rumrada  Meeboonya; Orawan  Wonganan; Pimolwan  Siripru; Panyada  Panyatip; Ploenthip  Puthongking

Chawalit Yongram Suan Sunandha Rajabhat University
Panupan Sripan Suan Sunandha Rajabhat University
Suwadee Chokchaisiri Suan Sunandha Rajabhat University
Rumrada Meeboonya Suan Sunandha Rajabhat University
Orawan Wonganan Suan Sunandha Rajabhat University
Pimolwan Siripru Melatonin Research Group, Faculty of Pharmaceutical Sciences, Khon Kaen University
Panyada Panyatip Division of Pharmacognosy, Faculty of Pharmacy, Srinakharinwirot University
Ploenthip Puthongking Division of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Khon Kaen University

Keywords: Phytochemical, Antioxidant, Inthajaworn, HPLC, GCMS

Abstract

The medicinal plant is a source of bioactive compounds and represents an alternative
used for the treatment of various diseases. In Thailand, there are several traditional medicine
recipes such as Bhamrung-Lohit, Ya-Ha-Rak and Inthajaworn recipe. These recipes were used
for treating many diseases such as antioxidant and anti- inflammatory activities, promoting
strength and prolonging the lifespan. In this study, the Inthajaworn recipe was determined the
chemical composition by HPLC and GCMS techniques and antioxidant capacity was analyzed
by DPPH and ABTS assays. The correlation between chemical content and antioxidant capacity
was analyzed. The result showed that the 6 phenolic and 2 flavonoid compounds were found in
Inthajaworn recipe which is quercetin showed the highest content (239.88±0.43 μg/g extract).
While the GCMS result was classified the chemical composition into 6 groups which are
Alkaloids (55.71%), Miscellaneous (17.30%), Triterpenes (7.61%), Long chain hydrocarbons
(3. 14% ) , Cannabinoids ( 1. 36% ) and Sesquiterpenes ( 0. 38% ) group. Piperine was the highest
abundant (48.08%). the Inthajaworn recipe presents the antioxidant capacity with DPPH and
ABTS values of 26.91±0.24 and 55.25±1.82 mg TE/g extract, respectively. In addition, Pearson
correlation analysis presented syringic acid, rutin and p-hydroxybenzoic acid were contributors
to antioxidant capacity (DPPH and ABTS). Therefore, these result findings suggest that the
Inthajaworn recipe is a source of natural antioxidant agents that could be used as an indicator
of the antioxidant properties in the Inthajaworn recipe.