THE DEVELOPMENT OF AN INNOVATIVE SUNSCREEN GEL FROM THE ACANTHUS EBRACTEATUSVAHLEXTRACT PRODUCT TO PREVENT OXIDATION AND ESTABLISH A COMMUNITY SPA FOR SUSTAINABILITY MANAGEMENT AT COLLEGE OF ALLIED HEALTH SCIENCES, SUAN SUNANDHA RAJABHAT UNIVERSITY, SAMUT
Abstract
This research focuses on the development of an innovative sunscreen gel using extracts
from Acanthus ebracteatus Vahl. The study has several objectives. Firstly, it aims to develop
natural extracts from Acanthus ebracteatus Vahl. Secondly, it intends to investigate the
antioxidant activity, anti-inflammatory effect, antimicrobial effect, and total phenolic content
of the extracts. Thirdly, the research examines the stability of the product. Finally, the study
aims to determine suitable formulations for the sunscreen gel using the Acanthus ebracteatus
Vahl extracts.
The research begins with the selection of raw materials and the preparation of extracts
to determine the total phenolic content. The antioxidant activity is assessed using the DPPH
assay method, while the anti-inflammatory effect is evaluated through tests such as nitric oxide
production inhibitory activity, inhibition of LPS-induced TNF-α secretion, and COX-2 anti-
inflammatory activity.
Once a suitable formulation is developed, tests are conducted to ensure the safety and
physical properties of the sunscreen gel product. This includes a skin irritation test involving
10 volunteers to assess its effectiveness and safety. The results of the product stability study
indicate that the sunscreen gel containing Acanthus ebracteatus Vahl extracts does not exhibit
phase separation. Furthermore, the irritation test on the volunteers after 12 hours of product use
shows no allergic reactions, itching, or irritation.
In addition, toxicity tests confirm the absence of cytotoxicity. The anti-inflammatory
effect test demonstrates potent anti-inflammatory properties when tested on RAW 264.7
macrophage cells. It is important to note that this study solely focuses on the development of
an innovative sunscreen gel using extracts from Acanthus ebracteatus Vahl and does not cover
other aspects of skincare or cosmetic products.
