Anti-Inflammatory Effects of Cassia alata L. in Human Keratinocytes

Jatupat  Anuchon; Wannee  Promdao; Narin  Kakatum; Supatta  khatippatee; Thitiya  Phommawanna

Jatupat Anuchon Applied Thai Traditional Medicine Program, College of Allied Health Sciences, Suan Sunandha Rajabhat University, Samut Songkhram 75000, Thailand
Wannee Promdao Applied Thai Traditional Medicine Program, College of Allied Health Sciences, Suan Sunandha Rajabhat University, Samut Songkhram 75000, Thailand
Narin Kakatum Applied Thai Traditional Medicine Program, College of Allied Health Sciences, Suan Sunandha Rajabhat University, Samut Songkhram 75000, Thailand
Supatta khatippatee Thai Traditional Medicine and Integrative Medicine Hospital, College of Allied Health Sciences, Suan Sunandha Rajabhat University, Samut Songkhram 75000, Thailand
Thitiya Phommawanna Applied Thai Traditional Medicine Program, College of Allied Health Sciences, Suan Sunandha Rajabhat University, Samut Songkhram 75000, Thailand

Keywords: Cassia alata L, keratinocytes, inflammation

Abstract

This study aimed to evaluate the antioxidant activity, cytotoxicity, and anti -inflammatory effects of Cassia alata L. leaf extract in human keratinocyte (HaCaT). Cytotoxicity determined by the resazurin assay revealed that the extract exhibited low toxicity at concentrations below 100 µg/mL, with cell viability remaining above 80-90%. Antioxidant evaluation using the DPPH assay demonstrated moderate radical scavenging activity, with an IC 50 value of 77.95 µg/mL. The anti-inflammatory effects assessed through the nitric oxide assay, expressed as normalized % of LPS, indicated that the 12.5 µg/mL extract significantly inhibited LPS-induced nitric oxide production, reducing nitrite levels to approximately 0 -5% of LPS, comparable to dexamethasone (100 nM ). The 25 µg /mL extract also significantly reduced NO production, although to a lesser extent. These findings suggest that C. alata leaf extract possesses promising anti-inflammatory potential with acceptable cellular safety pro files, supporting its application in anti-inflammatory herbal preparations or skin-related cosmeceutical formulations.