Antioxidant and Chemical contents of Thai Traditional Cannabis Recipes: Sanan Tripop oil

Sasipen  Krutchangthong; Worrapat  Saweksoot; Panupan  Sripan; Pradubphet  Krutchangthong; Chawalit  Yongram

Sasipen Krutchangthong Division of Applied Thai Traditional Medicine, College of Allied Health Sciences, Suan Sunandha Rajabhat University, Samut Songkhram 75000, Thailand
Worrapat Saweksoot Division of Cannabis Health Sciences, College of Allied Health Sciences, Suan Sunandha Rajabhat University, Samut Songkhram 75000, Thailand
Panupan Sripan Division of Aesthetic Health Sciences, College of Allied Health Sciences, Suan Sunandha Rajabhat University, Samut Songkhram 75000, Thailand
Pradubphet Krutchangthong Regulatory Technical Support Division, Office of Atoms for Peace, Bangkok 10900, Thailand
Chawalit Yongram Division of Applied Thai Traditional Medicine, College of Allied Health Sciences, Suan Sunandha Rajabhat University, Samut Songkhram 75000, Thailand

Keywords: Sanan Tripop oil, Antioxidant, Chemical contents

Abstract

The present study investigated the chemical composition and antioxidant activity of the Sanan Tripop oil recipe, a traditional Thai medicinal preparation historically used for the treatment of Kasai Lek, a condition characterized by abdominal pain, rigidit y, and digestive dysfunction . The oil was prepared from 17 medicinal plants, including Cannabis sativa, Ocimum spp., and Boesenbergia rotunda, extracted in sesame oil. Antioxidant activity was assessed using DPPH and ABTS radical scavenging assays and ferric reducing ability power (FRAP) assay, while phytochemical constituents, including total phenolics, flavonoids, chlorophylls, and carotenoids, were quantitatively analyzed. The results demonstrated weak antioxidant activity, with no observable effect in the DPPH assay (IC50 >1000 µg/mL) and low activity in the ABTS assay (IC50 = 825.04±24.65 µg/mL), also, FRAP value is 13 .24±0.78 mmol Fe 2+/100 g extract . Phytochemical analysis revealed total phenolics of 29 .38±1.15 mg GAE /g extract, total flavonoids of 90 .40±0.54 mg QE /g extract, total chlorophylls of 0 .08±0.00 mg/g extract, and total carotenoids of 0.03±0.00 mg/g extract. In conclusion, although the Sanan Tripop oil recipe exhibits limited free radical scavenging activity, its phytochemica l profile indicates potential for other bioactive effects, supporting the need for further pharmacological and in vivo investigations to clarify its therapeutic applications in integrative medicine.